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Historically, these characters were frequently used as comedic devices or plot twists. In older series, the reveal of a character's non-conforming gender was often played for shock value or "trap" tropes. However, as the global anime audience has expanded and matured, the top-tier examples of these characters have shifted toward more nuanced portrayals. Characters like Ferris Argyle from Re:Zero or Lily Hoshikawa from Zombie Land Saga demonstrate a move toward treating gender identity with a level of sincerity that transcends mere visual gags.

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